Read Online Dynamic Imaging of Secretion From Pancreatic Beta-cells by Confocal Fluorescence Microscopy - Weijun Qian | ePub
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The cpepsfgfp reporter mouse line allows the in vivo imaging of insulin secretion from individual islets after a glucose stimulation, in live, anesthetized mice.
Paneth cell granule secretion toward the lumen of the enteroids was evaluated by visualization and quanti˜ cation using high-speed 3d time-lapse imaging with a high-speed resonant scanner and a piezo z stage (fig. Enteroid secretion of granules rich in α-defensin microbiota food crypt small intestinal epithelium lumen intestinal microbiota.
This shows the power of our real-time imaging system, which allows the dynamic analysis with improved time resolution as well as analysis for temporal cell-to-cell variations in protein secretion. The concave-down increase of the il-1β secretion signal also suggested a burst release, rather than continuous secretion of il-1β.
Benefitting from these advantages, flp-(11–13, 19) are applied successfully in tracking the dynamic nature of lds and accumulation of lds in both aqueous solution and living cells, 3d imaging of lds for visualization of their repartition within the cells, and visualizing lds in tissues of diseases mice models including adipose, skeletal.
Dynamic imaging of ampa receptor trafficking in vitro biochemical, cell biological, and electrophysiological tools have been extensively used to study synaptic plasticity and have greatly advanced our knowledge of the underlying cellular and molecular mechanisms. However, optical microscopy allows for direct observation of cells and molecules.
Magnetic resonance imaging showed no lesion, but dynamic mri detected these small tumors may be clinically important because of secretion of hormones.
Dynamic photoacoustic imaging of neurovascular coupling in salivary glands. (a) schematic illustration of the dynamic pai method developed for temporal mapping of neurovascular dynamics in murine salivary glands. The method involves acquisition of a series of pa images before and after parasympathetic stimulation using pilocarpine.
Maximum output of pancreatic fluid is optimal between 6 to 8 minutes. A dynamic image and video is created from a 3-d rendering by the radiologist.
Dynamic imaging of coherent sources (dics) uses a spatial filter to localize coherent brain regions and provides the time courses of their activity. Reference points for the computation of neural coupling may be based on brain areas of maximum power or other physiologically meaningful information, or they may be estimated starting from sensor.
Thus, we performed real-time imaging on transgenic flies containing gfp-labeled secretory cargo (sgs3-gfp), gfp-tagged myosin ii (zipper-gfp), and rfp-tagged actin binding protein (lifeact-rfp) to visualize the dynamics of regulated secretion. Our imaging revealed apical and luminal expansion of the salivary glands, as sgs3-gfp is released from secretory granules in response to the hormone ecdysone.
Dynamic imaging methods are needed to follow the fate of cargo proteins and their interactions with resident enzymes and folding assistants. Here we applied the halotag labelling system to study the behavior of proteins with different fates and roles in esc: a chaperone, an erad substrate and an aggregation-prone molecule.
Visualization of the serratia type vi secretion system reveals unprovoked attacks and dynamic assembly graphical abstract highlights d t6sss are used by bacteria to attack competitors d fluorescence microscopy has been used to visualize a functioning t6ss in vivo d serratia t6ss fires without a cell contact trigger, targeting even peaceful.
Agonist stimulation of fluid secretion is initially associated with a rapid tenfold increase in [ca 2+] i as well as a substantial cell shrinkage. Subsequent changes of cell volume in the continued presence of agonist are tightly coupled to dynamic levels of [ca 2+] i, even during [ca 2+] i oscillations.
Describe the use of fluorescence microscopy to visualize the bacterial type vi secretion system in vivo. They show that serratia can indiscriminately fire this system without a trigger from a neighboring cell and observe distinct and dynamic behaviors of different core components of the system.
Dynamic mobile imaging (dmi) is locally owned and operated in richmond, virginia. The staff at dmi has extensive experience, including over 70 years combined in the field of radiology; 50 years in sales and marketing, and 15 years in technology.
Secretion, beta cell imaging introduction the islet of langerhans plays essential roles in controlling metabolism and glucose homeostasis through the release of peptide hormones. The islet beta cell, in particular, is crucial for maintaining euglycemia via insulin secretion. In healthy subjects, insulin secretion is tightly regulated, and beta.
With the development of synthetic human secretin (chirhostim®), gastroenterologists and radiologists are able to perform dynamic imaging of the pancreas. Smrcp enables providers to gain a better visualization of the main duct and side branchs of the pancreas and to examine exocrine pancreatic function.
Cell metastasis is a highly dynamic process that occurs in multiple steps. Understanding this process has been limited by the inability to visualize tumor cell behavior in real time by using animal models. Here, we employ translucent zebrafish and high-resolution confocal microscopy to study how human cancer cells invade in tissues, induce angiogenesis, and interact with newly formed vessels.
Biological membranes are highly dynamic and complex lipid bilayers, responsible for the fate of living cells. To achieve this function, the hydrating environment is crucial. However, membrane imaging typically neglects water, focusing on the insertion of probes, resonant responses of lipids, or the hydrophobic core.
A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells. Small extracellular vesicles called exosomes affect multiple autocrine and paracrine cellular phenotypes. Understanding the function of exosomes requires a variety of tools, including live imaging. A previous live-cell reporter, phluorin-cd63, allows dynamic subcellular monitoring of exosome secretion in migrating and spreading cells.
On the other hand, a comprehensive dynamic model involving secretion and detection dynamics is necessary to understand this complex process and precisely quantify the cell secretion [101, 102]. The real-time imaging process requires highly specific and selective responses from sensors.
Pd dr frank kirchhoff, cells also secrete biomolecules into the media.
Imaging of dynamic secretory vesicles in living pollen tubes of picea meyeri using evanescent wave microscopy.
3 oct 2014 dynamic imaging methods are needed to follow the fate of cargo proteins and their interactions with resident enzymes and folding assistants.
28 feb 2019 the value of pharmacokinetic parameters derived from dynamic contrast‑ enhanced magnetic resonance imaging (dce‑mri) in tumor characteristics and secretion types were determined through a combination of clinical.
Insulin secretion is a highly dynamic process regulated by various factors including nutrients, hormones, and neuronal inputs. The dynamics of insulin secretion can be studied at different levels: the single β cell, pancreatic islet, whole pancreas, and the intact organism.
22 mar 2013 atomic force microscopy: high resolution dynamic imaging of cellular to investigate: (1) fundamental issues in cell biology (secretion) and,.
Experimental calcium traces are used as inputs in three increasingly complex models of pool dynamics, which are fitted to insulin secretory patterns obtained using a set of protocols of glucose and tolbutamide stimu-lation. New calcium and secretion data for so-called staircase proto-.
Arrays [15, 17] and bead-based molecular sensors [22–24] for detecting cytokine secretion dynamically without the need for encapsulation of single t cells/nk cells. We used this meth-odology to link the phenotype of peripheral blood human nk cells with their dynamic cyto-kine secretion profiles.
The endoplasmic reticulum (er) plays a pivotal role in the regulation of cytosolic ca(2+) concentrations ([ca(2+)](cyt)) and hence in insulin secretion from pancreatic beta-cells. However, the molecular mechanisms involved in both the uptake and release of ca(2+) from the er are only partially defin.
In mammals, imprinted genes regulate many critical endocrine processes such as growth, the onset of puberty and maternal reproductive behaviour. Human imprinting disorders (ids) are caused by genetic and epigenetic mechanisms that alter the expression dosage of imprinted genes. Due to improvements in diagnosis, increasing numbers of patients with ids are now identified and monitored across.
24 aug 2017 phenotyping and real-time protein secretion of individual cells in a high- throughput manner.
Radiopharmaceuticals are available for functional renal imaging. However the dynamic renal scan shows no uptake or secretory phase in severe stenosis.
Pilocarpine increased salivary gland oxygen saturation (%so2) within minutes after administration, which was abrogated by atropine. A significant correlation was observed between change in %so2 measured by pai and saliva secretion. Pai is a novel imaging method that can be used for functional assessment of neurovascular dynamics in salivary glands.
The dynamic events of receptor activation or deactivation, even conformational change, can be accessed by receptor fluorescence resonance energy transfer (fret) and bioluminescence resonance energy transfer (bret) in a near-native system however, fret may suffer from time-dependent decreases in signal intensity due to exposure to incident.
22 apr 2014 real-time secretion imaging on the platform at 1-min time intervals the power of our real-time imaging system, which allows the dynamic.
21 dec 2016 dynamic imaging of allogeneic adipose-derived regenerative cells to prove that adrcs secrete a certain amount of paracrine factors,.
Understanding the function of exosomes requires a variety of tools, including live imaging. Our previous live-cell reporter, phluorin-cd63, allows dynamic subcellular monitoring of exosome.
Tem imaging provided ultrastructural detail of the subcellular architecture of the endomembrane system and served as a correlative tool to complement the flm and clsm imaging of live cells. Imaging of cross-sections reveals 16 cytoplasmic valleys with an average depth of 5–6 μm (measured from the base of a valley to the cortical cytoplasm.
Current techniques to study exosome physiology are based on isolation procedures after secretion, precluding direct and dynamic insight into the mechanics of exosome biogenesis and the regulation of their release. In this study, we propose real-time visualization of mvb–pm fusion to overcome these limitations.
Iris van den heuvel 16 series, is dedicated to dynamic renal imaging there is little or no secretion of 99mtc- dtpa.
11 apr 2019 single-cell dynamic imaging can also reveal new phenomena that can be one strategy for studying single cell protein secretion is to use total.
Many bacterial pathogens use a type iii secretion system (t3ss) to manipulate host cells. Protein secretion by the t3ss injectisome is activated upon contact to any host cell, and it has been.
Advances in fluorescence microscopy now allow for the direct visualization of the protein secretion process, detailing the dynamics of (i) outer membrane biogenesis and the assembly of protein secretion systems into the outer membrane, (ii) the spatial distribution of these and other membrane proteins on the bacterial cell surface, and (iii) translocation of effector proteins, toxins and enzymes by these protein secretion systems.
Appropriate insulin secretion is essential for maintaining euglycemia, and impairment or loss of insulin release represents a causal event leading to diabetes. There have been extensive efforts of studying insulin secretion and its regulation using a variety of biological preparations, yet it remains challenging to monitor the dynamics of insulin secretion at the cellular level in the intact.
Fluorescent labeling and dynamic imaging of secretory vesicles are new powerful means to study the mechanisms of protein and membrane trafficking.
The researchers used the imaris channel arithmetic matlab function to subtract the glandular signal (bottom) from the total epithelial signal (top).
Dynamic mr pancreatography exam performed following the administration of that simulates the hormone that causes liver and pancreas secretion.
Real-time secretion imaging on the platform at 1-min time intervals allowed successful detection of the heterogeneous onset time of nonclassical il-1β secretion from monocytes after external.
Imaging dynamic insulin release using a fluorescent zinc indicator for monitoring induced exocytotic release (zimir). Proceedings of the national academy of sciences 2011 108 (52) 21063-21068.
Production and secretion of hormones by the pituitary involve highly orchestrated intracellular transport and sorting steps. Hormone precursors are routed through a series of compartments before being packaged in secretory granules. These highly dynamic carriers play crucial roles in both pro-hormone processing and peptide exocytosis.
Our work here has thus expanded the use of gezis to image dynamic zn 2+ secretion in live tissue. Because it is convenient to use genetically encoded indicators for expression over extended periods and for in vivo delivery, we envision future applications of zibg2 in development of induced β-cells or islets to advance cell replacement therapies for diabetes and in direct imaging of zn 2+ secretion dynamics in vivo.
Dynamic imaging of pancreatic nuclear factor κb (nf-κb) activation in live mice the development of secreted luciferases such as gaussia (gluc), secreted.
Centre for dynamic imaging walter and eliza hall institute of medical research. Optical microscopy is one of the most powerful tools in medical research.
A significant correlation was observed between change in %so 2 measured by pai and saliva secretion. Pai is a novel imaging method that can be used for functional assessment of neurovascular dynamics in salivary glands.
Evanescent wave excitation was used to visualize individual, fm4-64-labeled secretory vesicles in an optical slice proximal to the plasma membrane of picea.
3 oct 2014 as a consequence, disturbances in these processes underlie many genetic and chronic diseases.
Dynamic imaging systems is a recognized leader in providing innovative software solutions to the law enforcement and correctional community. We incorporate the highest quality functionality into our products and integrate them into your jail management, records management and livescan systems.
31 aug 2015 dynamic µ-spect imaging to modelling of biodistribution in [1], with temporal estimation of uptake or secretion of 99mtc- pertechnetate after.
Temporal information about the release was traced out with a dynamic stimulating method by pumping secretagogue continuously passing the cell with the aid of electroosmotic flow and measuring the peak broadening of the released catecholamines in the electropherogram;direct visualization of the secretion process of individual bovine adrenal chromaffin cells was achieved by linf imaging microscopy with high temporal and spatial resolution.
Dynamic imaging of secretion from pancreatic beta-cells by confocal fluorescence microscopy: qian, weijun: amazon.
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